10.6084/m9.figshare.12027501.v1
Matheus Scarpatto Rodrigues
Matheus Scarpatto
Rodrigues
Bruno Luiz da Silva Pieri
Bruno Luiz da Silva
Pieri
Gustavo de Bem Silveira
Gustavo de Bem
Silveira
Rubya Pereira Zaccaron
Rubya Pereira
Zaccaron
Ligia Milanez Venturini
Ligia Milanez
Venturini
Vitor Hugo Comin
Vitor Hugo
Comin
Karine Damian Luiz
Karine Damian
Luiz
Paulo Cesar Lock Silveira
Paulo Cesar Lock
Silveira
Reduction of oxidative stress improves insulin signaling in cardiac tissue of obese mice
SciELO journals
2020
Obesity
Insulin resistance
Oxidative stress
Myocardium
Mice
2020-03-25 02:43:28
Dataset
https://scielo.figshare.com/articles/dataset/Reduction_of_oxidative_stress_improves_insulin_signaling_in_cardiac_tissue_of_obese_mice/12027501
<div><p>ABSTRACT Objective To evaluate the effects of oxidative stress on insulin signaling in cardiac tissue of obese mice. Methods Thirty Swiss mice were equally divided (n=10) into three groups: Control Group, Obese Group, and Obese Group Treated with N-acetylcysteine. After obesity and insulin resistance were established, the obese mice were treated with N-acetylcysteine at a dose of 50mg/kg daily for 15 days via oral gavage. Results Higher blood glucose levels and nitrite and carbonyl contents, and lower protein levels of glutathione peroxidase and phosphorylated protein kinase B were observed in the obese group when compared with their respective control. On the other hand, treatment with N-acetylcysteine was effective in reducing blood glucose levels and nitrite and carbonyl contents, and significantly increased protein levels of glutathione peroxidase and phosphorylated protein kinase B compared to the Obese Group. Conclusion Obesity and/or a high-lipid diet may result in oxidative stress and insulin resistance in the heart tissue of obese mice, and the use of N-acetylcysteine as a methodological and therapeutic strategy suggested there is a relation between them.</p></div>