%0 Generic %A Rodrigues, Matheus Scarpatto %A Pieri, Bruno Luiz da Silva %A Silveira, Gustavo de Bem %A Zaccaron, Rubya Pereira %A Venturini, Ligia Milanez %A Comin, Vitor Hugo %A Luiz, Karine Damian %A Silveira, Paulo Cesar Lock %D 2020 %T Reduction of oxidative stress improves insulin signaling in cardiac tissue of obese mice %U https://scielo.figshare.com/articles/dataset/Reduction_of_oxidative_stress_improves_insulin_signaling_in_cardiac_tissue_of_obese_mice/12027501 %R 10.6084/m9.figshare.12027501.v1 %2 https://scielo.figshare.com/ndownloader/files/22100418 %2 https://scielo.figshare.com/ndownloader/files/22100424 %2 https://scielo.figshare.com/ndownloader/files/22100427 %2 https://scielo.figshare.com/ndownloader/files/22100430 %K Obesity %K Insulin resistance %K Oxidative stress %K Myocardium %K Mice %X

ABSTRACT Objective To evaluate the effects of oxidative stress on insulin signaling in cardiac tissue of obese mice. Methods Thirty Swiss mice were equally divided (n=10) into three groups: Control Group, Obese Group, and Obese Group Treated with N-acetylcysteine. After obesity and insulin resistance were established, the obese mice were treated with N-acetylcysteine at a dose of 50mg/kg daily for 15 days via oral gavage. Results Higher blood glucose levels and nitrite and carbonyl contents, and lower protein levels of glutathione peroxidase and phosphorylated protein kinase B were observed in the obese group when compared with their respective control. On the other hand, treatment with N-acetylcysteine was effective in reducing blood glucose levels and nitrite and carbonyl contents, and significantly increased protein levels of glutathione peroxidase and phosphorylated protein kinase B compared to the Obese Group. Conclusion Obesity and/or a high-lipid diet may result in oxidative stress and insulin resistance in the heart tissue of obese mice, and the use of N-acetylcysteine as a methodological and therapeutic strategy suggested there is a relation between them.

%I SciELO journals