10.6084/m9.figshare.5734359.v1
Hugo Teixeira Gomes
Hugo Teixeira
Gomes
Patrícia Monah Cunha Bartos
Patrícia Monah Cunha
Bartos
Maíra Teixeira de Andrade
Maíra Teixeira de
Andrade
Raphael Ferreira Almeida
Raphael Ferreira
Almeida
Luciana Florencio de Lacerda
Luciana Florencio de
Lacerda
Jonny Everson Scherwinski-Pereira
Jonny Everson
Scherwinski-Pereira
In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation
SciELO journals
2017
Rubus
ex situ conservation
plant genetic resources
plant propagation
slow-growth
2017-12-27 02:42:37
Dataset
https://scielo.figshare.com/articles/dataset/In_vitro_conservation_of_blackberry_genotypes_under_minimal_growth_conditions_and_subsequent_large-scale_micropropagation/5734359
<div><p>Abstract: The objective of this work was to evaluate the micropropagation of blackberry (Rubus spp.) cultivars, after in vitro conservation under minimal growth conditions. Nodal segments of the 'Guarani', 'Caingangue', 'Ébano', and 'Xavante' genotypes were conserved under minimal growth conditions at 20ºC, for 15 months. Microshoots were regenerated and multiplied by up to five successive subcultures, when they were rooted and acclimatized. After 30 days of acclimatization in a greenhouse, rooted plantlets showed no significant losses. Blackberry cultivars can be conserved in vitro for 15 months, without subcultures and, after this time, they can be micropropagated on a large-scale, maintaining the regenerative potential and multiplication.</p></div>