Cryopreservation of banana’s cv Grand Naine in vitro rhizomes LUCIANA C.N. LONDE WAGNER A. VENDRAME MASSY SANAEI ALEXANDRE B. DE OLIVEIRA 10.6084/m9.figshare.6124880.v1 https://scielo.figshare.com/articles/dataset/Cryopreservation_of_banana_s_cv_Grand_Naine_in_vitro_rhizomes/6124880 <div><p>ABSTRACT The preservation of banana genetic material is usually performed through seedlings. However, most banana cultivars do not produce seed and are propagated vegetatively. Therefore, cryopreservation is a feasible technique that allows the preservation of banana genotypes indefinitely. For the success of cryopreservation protocols, the selection of cryoprotectants and pre-freezing techniques are important factor. Therefore, the objective of this study was to verify the effects of different cryoprotectants with and without 1% phloroglucinol and pre-cooling periods on the development of a protocol for cryopreservation of in vitro rhizomes ofMusa accuminata(AAA) cv Grand Naine banana. The addition of 1% phloroglucinol to the cryoprotective solutions, such as PVS2 enhanced recovery of cryopreserved banana rhizomes. In addition, pre-cooling of explants in ice for 3 hours in PVS2 + 1% of phloroglucinol allowed efficient cryopreservation of banana rhizomes, followed by successful recovery and regeneration of in vitro shoots of banana cv Grand Naine.</p></div> 2018-04-11 02:46:12 Musa acumminata in vitro conservation cryoprotectants pre-cooling