%0 Generic %A TUMER, Mehmet Kemal %A CICEK, Mustafa %D 2018 %T Differential immunohistochemical expression of type I collagen and matrix metalloproteinase 2 among major salivary glands of young and geriatric mice %U https://scielo.figshare.com/articles/dataset/Differential_immunohistochemical_expression_of_type_I_collagen_and_matrix_metalloproteinase_2_among_major_salivary_glands_of_young_and_geriatric_mice/6503471 %R 10.6084/m9.figshare.6503471.v1 %2 https://scielo.figshare.com/ndownloader/files/11956253 %2 https://scielo.figshare.com/ndownloader/files/11956259 %2 https://scielo.figshare.com/ndownloader/files/11956265 %2 https://scielo.figshare.com/ndownloader/files/11956271 %2 https://scielo.figshare.com/ndownloader/files/11956274 %2 https://scielo.figshare.com/ndownloader/files/11956283 %2 https://scielo.figshare.com/ndownloader/files/11956289 %2 https://scielo.figshare.com/ndownloader/files/11956295 %K Parotid gland %K Type 1 collagen %K Salivary glands %K Matrix metalloproteinase 2 %K Aging %X

Abstract Objective This study aimed to demonstrate the immunohistochemical changes associated with MMP-2 and type 1 collagen separately for the first time in the major salivary glands (the parotid, submaxillary, and sublingual glands) that occur with aging in mice. Material and Methods Fourteen Balb/c white mice (50-80 g) were used in this study. The animals were divided into two equal groups. Group I consisted of young animals (2-month-old) (n=7) and Group II consisted of older animals (18-month-old) (n=7). After routine histological follow-ups, Hematoxylin-eosin (H&E), Masson’s Trichrome staining and immunohistochemical staining was performed for type I collagen and MMP-2. Results We observed that there were age-related decreases in the number of acinar cells, increase in eosinophilic zymogen granules in cells, collagen accumulation in fibrotic areas and dilatation in interlobular ducts. Also, while type I collagen and MMP-2 immunoreactivity were moderate in the salivary glands of the young mice, they were high in the salivary glands of the old mice (p=0.001). In the H-score assessment, MMP-2 immunoreactivity was lower at a significant level in young mice than in old mice (p=0.001). Conclusions This study showed that anatomical, physiological and morphological abnormalities occur in all three major salivary glands as a natural consequence of aging.

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