10.6084/m9.figshare.6857585.v1
Renato Fernandes Galdiano Júnior
Renato Fernandes
Galdiano Júnior
Cibele Mantovani
Cibele
Mantovani
Eliana Gertrudes de Macedo Lemos
Eliana Gertrudes de Macedo
Lemos
Carbohydrates on the growth of Cattleya schilleriana Reichb.f. seedlings (Orchidaceae)
SciELO journals
2018
in vitro culture
native orchid
glucose
sucrose
acclimatization
2018-07-25 02:53:20
Dataset
https://scielo.figshare.com/articles/dataset/Carbohydrates_on_the_growth_of_Cattleya_schilleriana_Reichb_f_seedlings_Orchidaceae_/6857585
<div><p>ABSTRACT: The aim of the present study was to evaluate the effects of carbohydrate supplementation on the propagation of the orchid Cattleya schilleriana. The 120-d-old seedlings were subcultured in fructose-, glucose-, or sucrose-supplemented (0, 15, 30, and 45g L-1) ½ MS culture medium (half-strength macronutrient concentrations), using a completely random design with four repetitions per treatment. After 120d of treatment, root number and length, leaf number and length, and fresh weight were evaluated, and seedling survival was evaluated after 75d of acclimatization in a greenhouse. The in vitro growth data were submitted to regression analysis, whereas the percentage survival data were analyzed using ANOVA and Tukey’s test. Both in vitro growth and ex vitro survival were lowest when the plantlets were grown in the absence of a carbohydrate source and highest (>90% survival) when supplemented with glucose. According to our findings, the addition of either glucose (30g L-1) or sucrose (30g L-1) is recommended for mass propagation of C. schilleriana.</p></div>