10.6084/m9.figshare.6991517.v1
Paulo Hernane Rabelo Azevedo
Paulo Hernane Rabelo
Azevedo
Marcelo Antônio Pascoal Xavier
Marcelo Antônio Pascoal
Xavier
Glenda Nicioli da Silva
Glenda Nicioli da
Silva
Priscilla Almeida da Costa
Priscilla Almeida da
Costa
Cláudia Martins Carneiro
Cláudia Martins
Carneiro
Geraldo Brasileiro Filho
Geraldo
Brasileiro Filho
Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection
SciELO journals
2018
Chagas disease
Heart transplantation
Immunohistochemistry
Trypanosoma cruzi
2018-08-22 02:42:21
Dataset
https://scielo.figshare.com/articles/dataset/Anti-serum_validation_for_use_in_immunohistochemistry_for_Trypanosoma_cruzi_detection/6991517
<div><p>Abstract INTRODUCTION: The detection of Trypanosoma cruzi in tissue samples is important in many situations, such as testing of the reactivation of the infection. The detection of T. cruzi nests in endomyocardial biopsies (EMB) may be useful to evaluate graft rejection. Given their scarcity, such nests are not routinely identified. To increase the diagnosis sensitivity, immunohistochemistry (IHC) may serve as a promising strategy. Here, we validate an antiserum for the detection of T. cruzi infection by IHC. METHODS: We used 1) positive controls (PCs) - 13 EMB, 12 skin biopsies, and 1 heart with T. cruzi nests as sections stained with hematoxylin and eosin (HE); 2) negative controls - a) 10 explant hearts and 10 EMB with no amastigote nests or clinical/laboratory signs of chagasic infection; and b) eight samples with leishmaniasis, toxoplasmosis, or histoplasmosis; and 3) Cases - 31 EMB of chagasic patients with no parasite nests in HE sections but detected positive for T. cruzi DNA by polymerase chain reaction. As a primary antibody, a hyperimmune serum from T. cruzi-infected rabbits was used. RESULTS: IHC results were positive for 21 of 26 PCs (80.8%) and one case of cutaneous leishmaniasis. In 4 of 31 cases, IHC revealed nests (12.9%), which were undetected by conventional histological examination. CONCLUSIONS: This study shows that IHC with the tested antiserum increases the sensitivity of the diagnosis and may be recommended for routine use in EMB analyses of cardiac transplant patients with Chagas disease.</p></div>