10.6084/m9.figshare.6992057.v1
Michelle M. A. C. Ribeiro
Michelle M. A. C.
Ribeiro
Mariana C. Marra
Mariana C.
Marra
Brenda M. C. Costa
Brenda M. C.
Costa
Thiago C. Oliveira
Thiago C.
Oliveira
Alex D. Batista
Alex D.
Batista
Rodrigo A. A. Muñoz
Rodrigo A. A.
Muñoz
Eduardo M. Richter
Eduardo M.
Richter
Sub-Minute Method for Determination of Naphazoline in the Presence of Diphenhydramine, Pheniramine or Chlorpheniramine by Capillary Electrophoresis
SciELO journals
2018
short length column
sub-minute separation
quality control
antihistamines
imidazole derivative
2018-08-22 02:49:30
Dataset
https://scielo.figshare.com/articles/dataset/Sub-Minute_Method_for_Determination_of_Naphazoline_in_the_Presence_of_Diphenhydramine_Pheniramine_or_Chlorpheniramine_by_Capillary_Electrophoresis/6992057
<div><p>This paper presents a simple and low-cost capillary electrophoresis method for ultra-fast simultaneous determination of naphazoline (NPZ) and one of the following active ingredients: diphenhydramine (DIP), pheniramine (PHEN) or chlorpheniramine (CPHEN). The proposed method is based on capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D) equipped with a short length capillary column (10 cm). One sample can be analyzed every 35 s (ca. 100 injections per hour) with resolutions between peaks greater than 1.4. The optimized background electrolyte (BGE) was composed by 20 mmol L-1 histidine and 10 mmol L-1 3,4-dimethoxycinnamic acid (pH = 9.5, adjusted with NaOH). Limits of detection were 25 µmol L-1 for NPZ, DIP, and PHEN and 13 µmol L-1 for CPHEN. The results obtained with the developed procedure were compared to those obtained by high-performance liquid chromatography (HPLC) and no statistically significant differences were observed (95% confidence level).</p></div>