10.6084/m9.figshare.7101278.v1 Rachel Lopes Rodrigues Gomes Rachel Lopes Rodrigues Gomes Rodrigo Galvão Viana Rodrigo Galvão Viana Luiz Alberto Soares Melo Jr Luiz Alberto Soares Melo Jr Alessandro Carvalho Cruz Alessandro Carvalho Cruz Acácio Alves de Souza Lima Filho Acácio Alves de Souza Lima Filho Eunice Mayumi Suenaga Eunice Mayumi Suenaga Mauro Campos Mauro Campos Aqueous humor concentrations of topical fluoroquinolones alone or in combination with a steroid SciELO journals 2018 Aqueous humor Ophthalmic solutions Antibiotic prophylaxis Fluoroquinolones Steroids Adrenal cortex hormones Anti-bacterial agents Comparative study 2018-09-19 02:45:38 Dataset https://scielo.figshare.com/articles/dataset/Aqueous_humor_concentrations_of_topical_fluoroquinolones_alone_or_in_combination_with_a_steroid/7101278 <div><p>ABSTRACT Purpose: To compare the aqueous humor (AH) concentrations of moxifloxacin 0.5% and gatifloxacin 0.3% solutions alone or when treatment was combined with steroids, and to correlate these concentrations with the minimum inhibitory concentrations (MIC) for the most common endophthalmitis-causing organisms. Methods: Patients undergoing phacoemulsification were enrolled to receive one drop of one of the following solutions: moxifloxacin (G1), moxifloxacin + dexamethasone (G2), gatifloxacin (G3), or gatifloxacin + c (G4), every 15 min, 1h before surgery. AH samples were collected before surgery and analyzed using HPLC-tandem mass spectrometry. Results: The mean antibiotic concentrations in the AH were: G1= 1280.8 ng/mL; G2= 1644.3 ng/mL; G3= 433.7 ng/mL; and G4= 308.1 ng/mL. The mean concentrations statistically differed between G1 and G2 (p=0.01), and G3 and G4 (p=0.008). All samples achieved the MIC for Staphylococcus epidermidis; 100% of the samples from G1 and G2, and 97% from G3 and G4 reached the MIC for fluoroquinolone-sensitive Staphylococcus aureus; 100% of the samples from G1 and G2, 88% from G3, and 72% from G4 reached the MIC for enterococci (p<0.001); and 100% of samples from G1 and G2, 59% from G3, and 36% from G4 reached the MIC for Streptococcus pneumoniae (p<0.001). For fluoroquinolone-resistant S. aureus, 23% from G1, 44% from G2, and no samples from G3 or G4 achieved the MIC (p<0.001). Conclusions: Moxifloxacin + dexamethasone demonstrated a higher concentration in the AH than the moxifloxacin alone. Gatifloxacin + steroids demonstrated less penetration into the anterior chamber than gatifloxacin alone. Moxifloxacin was superior to gatifloxacin considering the MIC for enterococci, S. pneumoniae, and fluoroquinolone-resistant S. aureus.</p></div>