%0 Generic %A Cabana, Ângela Leitzke %A Mendes, Josiara Furtado %A Baracy Klafke, Gabriel %A Brandolt, Tchana Martinez %A Melo, Aryse Martins %A Meireles, Mário Carlos Araújo %A Xavier, Melissa Orzechowski %D 2018 %T Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test? %U https://scielo.figshare.com/articles/dataset/Can_Aspergillus_fumigatus_conidia_cause_false-positive_results_in_the_galactomannan_enzyme_immunoassay_test_/7452386 %R 10.6084/m9.figshare.7452386.v1 %2 https://scielo.figshare.com/ndownloader/files/13797488 %2 https://scielo.figshare.com/ndownloader/files/13797491 %K Invasive aspergillosis %K Diagnosis %K Platelia® Aspergillus EIA %K Galactomannan %K Air quality %X

Abstract INTRODUCTION: Several factors can cause false-positive results in the galactomannan (GM) test; however, others remain unknown. Presently, the impact of airborne contamination by Aspergillus conidia during enzyme-linked immunosorbent assay (ELISA) remains uninvestigated. METHODS: We studied 12 A. fumigatus isolates. Fungal conidia were serially diluted and tested for GM detection using the Platelia® Aspergillus enzyme immunoassay (EIA). RESULTS: The conidia concentration required for an EIA-positive result was 4.8 × 103 (median). CONCLUSIONS: This is the first study to evaluate the impact of environmental contamination on the Platelia® Aspergillus EIA assay. Only massive contamination can interfere with GM optical readings, suggesting that environmental contamination does not cause false-positive test results.

%I SciELO journals