Occurrence of Mycoplasma bovigenitalium and Ureaplasma diversum in dairy cattle from to Pernambuco state, Brazil A.A.M. Macêdo J.M.B. Oliveira B.P. Silva J.M. Borges L.B.F. Soares G.M. Silva S.B. Santos R.A. Mota J.W. Pinheiro-Júnior 10.6084/m9.figshare.7482245.v1 https://scielo.figshare.com/articles/dataset/Occurrence_of_Mycoplasma_bovigenitalium_and_Ureaplasma_diversum_in_dairy_cattle_from_to_Pernambuco_state_Brazil/7482245 <div><p>ABSTRACT The objective of this study was to conduct an investigation of Mycoplasma bovigenitalium and Ureaplasma diversum infections in cattle in the microregion of the Ipanema Valley, state of Pernambuco, Brazil. Vaginal swabs were collected from 355 breeding cows in reproductive age and were analyzed by multiplex PCR (mPCR) and culture. An epidemiological investigation of risk factors was performed for Mollicutes. mPCR analysis showed that, 9.29% (33/355) of the cows were positive for M. bovigenitalium and 21.69% (77/355) for U. diversum; coinfection was observed in 2.81% (10/355) of the cows. The microbiological isolation showed, 81.81% (27/33) of Mycoplasma spp. and 24.67% (19/77) of Ureaplasma spp.. The risk factors related to Mollicutes infection identified were semi-intensive breeding system (OR= 4.6), pasture rent (OR= 3.6), non-isolation of animals with reproductive disorders (OR= 3.2), and natural mounting and artificial insemination (OR= 3.5). There was a significant association between Mollicutes infection and abortions in the first gestational third (P= 0.001). This is the first record of M. bovigenitalium and U. diversum infection in cows in the semiarid region of the state of Pernambuco, Brazil. Preventive measures directed to the identified risk factors can decrease the occurrence of Mollicutes in these herds.</p></div> 2018-12-19 02:46:16 mPCR mollicutes cows risk factors ipanema valley