10.6084/m9.figshare.7507379.v1
Nathálya dos Santos Martins
Nathálya dos Santos
Martins
Glaucia Barbosa Coelho
Glaucia Barbosa
Coelho
Larissa Sarmento dos Santos
Larissa Sarmento dos
Santos
Rudson Almeida de Oliveira
Rudson Almeida de
Oliveira
Ana Lúcia Abreu Silva
Ana Lúcia Abreu
Silva
Ferdinan Almeida Melo
Ferdinan Almeida
Melo
CHANGES IN THE SPLENIC EXTRACELLULAR MATRIX IN DOGS NATURALLY INFECTED WITH Leishmania (Leishmania) infantum chagasi
SciELO journals
2018
extracellular matrix
Leishmania
spleen
2018-12-26 02:59:06
Dataset
https://scielo.figshare.com/articles/dataset/CHANGES_IN_THE_SPLENIC_EXTRACELLULAR_MATRIX_IN_DOGS_NATURALLY_INFECTED_WITH_Leishmania_Leishmania_infantum_chagasi/7507379
<p></p><p>The aim of this study was to study the changes in the splenic extracellular matrix of dogs naturally infected with Leishmania (Leishmania) infantum chagasi and its correlation to clinical, histopathological, and parasitological aspects. Eighteen dogs were used, separated into three groups: six non-infected animals (control group) and twelve infected animals. The dogs had undefined breed and age, from the township region of São Luís-MA. Paraffined slices of the spleen were stained with Hematoxilin and Eosin (H&E); Gomori’s ammoniacal Silver, to mark reticular fibers; and the Immunohistochemistry technique of streptavidin peroxidase to detect amastigote forms of Leishmania. The morphometrical analyses were done using the KS300 program and the images analysis system Kontron Elektronic/Carl Zeiss, Germany. The results showed that there is a significant increase in the deposition of collagen fibers in the spleen, compared to control animals, showing significant differences for symptomatic and asymptomatic animals. Positive correlations were found between the presence of the parasite in the tissue and collagen deposition. Symptomatic animals showed higher collagen deposition in the spleen, which can be associated to the high parasitism found in the tissue. The results showed that there is an intense fibrogenesis in the spleen in the canine visceral leishmaniasis, thus being associated to the parasitism of the tissue and the degenerative processes of the disease.</p><p></p>