10.6084/m9.figshare.7517924.v1
Teresa Cristina P. Bonanséa
Teresa Cristina P.
Bonanséa
Monique Nakayama Ohe
Monique Nakayama
Ohe
Cynthia Brandão
Cynthia
Brandão
Cláudia de Francischi Ferrer
Cláudia de Francischi
Ferrer
Lívia Marcela Santos
Lívia Marcela
Santos
Marise Lazaretti-Castro
Marise
Lazaretti-Castro
José Gilberto Henriques Vieira
José Gilberto Henriques
Vieira
Experience with a third-generation parathyroid hormone assay (BIO-PTH) in the diagnosis of primary hyperparathyroidism in a Brazilian population
SciELO journals
2018
Primary hyperparathyroidism
Bio-PTH
intact PTH
PTH assays
2018-12-26 05:57:01
Dataset
https://scielo.figshare.com/articles/dataset/Experience_with_a_third-generation_parathyroid_hormone_assay_BIO-PTH_in_the_diagnosis_of_primary_hyperparathyroidism_in_a_Brazilian_population/7517924
<div><p>ABSTRACT Objective To evaluate the usefulness of a third-generation PTH assay in the diagnosis of primary hyperparathyroidism (PHPT). Subjects and methods Forty-one PHPT patients (4 men and 37 women) with 61.2 ± 10.9 (mean ± SD) years, were studied and had PTH levels measured with two different methods using the same immunochemiluminescent assay plataform (Elecsys 2010 System, Roche). We compared a second-generation assay (I-PTH) with a third-generation PTH assay (Bio-PTH). Two populations of 423 and 120 healthy adults with serum 25OHD levels above 25 ng/mL were used to define normal values in the I-PTH and Bio-PTH assays respectively. Results Normal PTH values based in the healthy adults population were 24.2-78.0 pg/mL for the I-PTH assay and 19.9-58.5 pg/mL for Bio-PTH assay. In PHPT patients, PTH values ranged from 67 to 553 pg/mL (median: 168 pg/mL) using the I-PTH assay and from 55 to 328 pg/mL (median: 111 pg/mL) using the Bio-PTH assay. Results obtained with the Bio-PTH assay were significantly lower (p < 0.0001, Wilcoxon). In general I-PTH and Bio-PTH showed highly significant correlation (r = 0.952, p < 0.0001). Passing–Bablok analysis gave a regression equation of Bio PTH = 13.44 + 0.59 x intact PTH. PHPT patients had 25OHD levels ranging from 4 to 36 ng/mL (mean 16.2 ng/mL); 35 subjects (85.3%) had values bellow 25 ng/mL. Conclusion Our results demonstrate that both second and third generation PTH methods are strongly correlated in PHPT patients and control subjects. Lower results with Bio-PTH tests are expected in function of the assay specificity determined by the amino-terminal antibody used.</p></div>