In vitro potency test for evaluation of Clostridium perfringens type D epsilon toxoid
Felipe Masiero Salvarini
Zélia Ines Portela Lobato
Prhiscylla Sadanã Pires
Rodrigo Otávio Silveira Silva
Guilherme Guerra Alves
Pedro Lúcio Lithg Pereira
Francisco Carlos Faria Lobato
10.6084/m9.figshare.7518401.v1
https://scielo.figshare.com/articles/dataset/In_vitro_potency_test_for_evaluation_of_Clostridium_perfringens_type_D_epsilon_toxoid/7518401
<div><p>The control of enterotoxemia caused by the epsilon toxin, produced by Clostridium perfringenstype D, is based on vaccination with epsilon toxoid. The potency test for this immunogen is conducted using seroneutralization in mice. Here, an in vitro test for detection of neutralizing antibodies with Madin-Darby Canine Kidney (MDCK) cells was standardized in order to study alternative methodologies for the potency test. Titers observed in the in vivo and in vitro seroneutralization tests had a correlation of 99.73%.</p></div>
2018-12-26 06:05:51
seroneutralization
mouse
cell culture
bioethics
vaccine