Pavan, T.R. Di Domenico, J. Kirsten, K.S. Nied, C.O. Frandoloso, R. Kreutz, L.C. Antibody response in silver catfish (Rhamdia quelen) immunized with a model antigen associated with different adjuvants <div><p>Adjuvants are essential to boost the immune response to inoculated antigen and play a central role in vaccine development. In this study, we investigated the efficacy of several adjuvants in the production of anti-bovine serum albumin (BSA) antibodies in silver catfish. Two hundred and seventy juvenile silver catfish (60–80 g) of both sexes were intraperitoneally vaccinated with BSA (200 µg/fish) alone or mixed to the following adjuvants: Freund’s complete adjuvant (FCA), Freund’s incomplete adjuvant (FIA), aluminum hydroxide (AlOH), Montanide, four types of cytosine-phosphate-guanine (CpG) oligodeoxynucleotides (ODNs) and three concentrations of β-glucan, and the immune enhancing property was evaluated by measuring anti-BSA antibodies in blood samples at biweekly intervals. Our results demonstrated that CpGs ODNs and β-glucan were as effective as classical adjuvants (FCA, FIA, AlOH and Montanide) in promoting anti-BSA antibodies and that the kinetics of antibody production induced by all adjuvants used in our study had a similar trend to that observed in other fish species, with a peak at 28 days post-vaccination. These results may be useful for the selection of adjuvants for vaccine formulation intended for silver catfish and for the development of vaccine and vaccination strategies to other fish species.</p></div> Vaccination;Fish;Immune response;CpGs ODN;β-glucan 2019-03-27
    https://scielo.figshare.com/articles/figure/Antibody_response_in_silver_catfish_Rhamdia_quelen_immunized_with_a_model_antigen_associated_with_different_adjuvants/7898960
10.6084/m9.figshare.7898960.v1