%0 Generic %A Cavalcante, Elisângela O. %A Suzuki, Lisandra A. %A Rossi, Claudio Lucio %D 2019 %T A quantitative enzyme-linked immunosorbent assay (ELISA) for the detection of anti-double-stranded DNA IgG antibodies %U https://scielo.figshare.com/articles/dataset/A_quantitative_enzyme-linked_immunosorbent_assay_ELISA_for_the_detection_of_anti-double-stranded_DNA_IgG_antibodies/8195063 %R 10.6084/m9.figshare.8195063.v1 %2 https://scielo.figshare.com/ndownloader/files/15272450 %K systemic lupus erythematosus %K antinuclear antibodies %K enzyme-linked immunosorbent assay %K indirect fluorescent antibody technique %X

ABSTRACT Introduction: The detection of anti-double-stranded (ds) deoxyribonucleic acid (DNA) antibodies is one of the classification criteria for diagnosing systemic lupus erythematosus (SLE). Objective: To describe a quantitative enzyme-linked immunosorbent assay (ELISA) for detecting anti-dsDNA immunoglobulin class G (IgG) antibodies. Methods: The performance of ELISA was evaluated using the Crithidia luciliae indirect immunofluorescence test (CLIFT) as a reference. Anti-dsDNA IgG antibodies were screened by ELISA and CLIFT in serum samples from 127 patients with SLE, 56 patients with other diseases and 37 healthy persons. The Cochran Q test was used to compare the sensitivity and specificity of the reactions, with differences among the results being considered significant when p ≤ 0.05. Results: ELISA had a sensitivity of 92.9% and a specificity of 94.6%, whereas the sensitivity and specificity of CLIFT were 85.8% and 100%, respectively. ELISA was significantly more sensitive than CLIFT (p = 0.0027), whereas CLIFT was significantly more specific than ELISA (p = 0.0253). Conclusion: ELISA showed excellent results in terms of sensitivity and specificity, with a potential use in research and routine diagnostics.

%I SciELO journals