10.6084/m9.figshare.8227025.v1 Danielle Brasiliense Danielle Brasiliense Rodrigo Cayô Rodrigo Cayô Ana Paula Streling Ana Paula Streling Carolina S Nodari Carolina S Nodari Rafael R Barata Rafael R Barata Poliana S Lemos Poliana S Lemos Janaina M Massafra Janaina M Massafra Yan Correa Yan Correa Igor Magalhães Igor Magalhães Ana C Gales Ana C Gales Roberta Sodré Roberta Sodré Diversity of metallo-β-lactamase-encoding genes found in distinct species of Acinetobacter isolated from the Brazilian Amazon Region SciELO journals 2019 metallo-β-lactamase non-baumannii Acinetobacter species polymyxin resistance nosocomial infection ICU Tn125 2019-06-05 02:46:37 Dataset https://scielo.figshare.com/articles/dataset/Diversity_of_metallo-_-lactamase-encoding_genes_found_in_distinct_species_of_Acinetobacter_isolated_from_the_Brazilian_Amazon_Region/8227025 <div><p> BACKGROUND The multidrug resistance (MDR) phenotype is frequently observed in Acinetobacter baumannii, the most clinically relevant pathogenic species of its genus; recently, other species belonging to the A. calcoaceticus-A. baumannii complex have emerged as important MDR nosocomial pathogens. OBJECTIVES The present study aimed to verify the occurrence of metallo-β-lactamase genes among distinct Acinetobacter species in a hospital located in the Brazilian Amazon Region. METHODS Antimicrobial susceptibility profiles were determined by broth microdilution. The genetic relationships among these isolates were assessed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Pyrosequencing reads of plasmids carrying the bla NDM-1 gene were generated using the Ion Torrent™ platform sequencing. FINDINGS A total of six isolates carried bla NDM-1: A. baumannii (n = 2), A. nosocomialis (n = 3), and A. pittii (n = 1); three carried bla IMP-1: A. baumannii, A. nosocomialis, and A. bereziniae. Resistance to colistin was observed for an NDM-1-producing A. nosocomialis isolate. Diverse PFGE patterns and sequence types were found among A. nosocomialis and A. baumannii isolates. The bla NDM-1 sequence was inserted in a Tn125 transposon, while the bla IMP-1 was found as a gene cassette of the class 1 integron In86. MAIN CONCLUSIONS To the best of our knowledge, this is the first report describing the dissemination of bla NDM-1 among distinct Acinetobacter species recovered from the same hospital in South America.</p></div>