10.6084/m9.figshare.9276533.v1
Patrícia Heloise Alves Bezerra
Patrícia Heloise Alves
Bezerra
Isadora Marques Ferreira
Isadora Marques
Ferreira
Beatriz Tinoco Franceschi
Beatriz Tinoco
Franceschi
Francine Bianchini
Francine
Bianchini
Luciana Ambrósio
Luciana
Ambrósio
Adélia Cristina O. Cintra
Adélia Cristina O.
Cintra
Suely Vilela Sampaio
Suely
Vilela Sampaio
Fabíola Attié de Castro
Fabíola Attié de
Castro
Maria Regina Torqueti
Maria Regina
Torqueti
BthTX-I from Bothrops jararacussu induces apoptosis in human breast cancer cell lines and decreases cancer stem cell subpopulation
SciELO journals
2019
apoptosis
bothropstoxin
breast cancer
cancer stem cells
2019-08-07 02:47:49
Dataset
https://scielo.figshare.com/articles/dataset/BthTX-I_from_Bothrops_jararacussu_induces_apoptosis_in_human_breast_cancer_cell_lines_and_decreases_cancer_stem_cell_subpopulation/9276533
<div><p>ABSTRACT Background: Breast cancer is the neoplasm with both the highest incidence and mortality rate among women worldwide. Given the known snake venom cytotoxicity towards several tumor types, we evaluated the effects of BthTX-I from Bothrops jararacussu on MCF7, SKBR3, and MDAMB231 breast cancer cell lines. Methods: BthTX-I cytotoxicity was determined via MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide assay. Cell death was measured by a hypotonic fluorescent solution method, annexin-V-FITC/propidium iodide staining and by apoptotic/autophagic protein expression. Cancer stem cells (CSCs) were quantified by flow cytometry using anti-CD24-FITC and anti-CD44-APC antibodies and propidium iodide. Results: BthTX-I at 102 µg/mL induced cell death in all cell lines. The toxin induced apoptosis in MCF7, SKBR3, and MDAMB231 in a dose-dependent manner, as confirmed by the increasing number of hypodiploid nuclei. Expression of pro-caspase 3, pro-caspase 8 and Beclin-1 proteins were increased, while the level of the antiapoptotic protein Bcl-2 was diminished in MCF7 cells. BthTX-I changed the staining pattern of CSCs in MDAMB231 cells by increasing expression of CD24 receptors, which mediated cell death. Conclusions: BthTX-I induces apoptosis and autophagy in all breast cancer cell lines tested and also reduces CSCs subpopulation, which makes it a promising therapeutic alternative for breast cancer.</p></div>