10.6084/m9.figshare.9697184.v1
João Maximiliano Pedron Martins
João Maximiliano Pedron
Martins
Fernanda dos Santos de Oliveira
Fernanda dos Santos de
Oliveira
Elizabeth Obino Cirne Lima
Elizabeth Obino Cirne
Lima
Diego Dullius
Diego
Dullius
Isabel Cirne Lima de Oliveira Durli
Isabel Cirne Lima de Oliveira
Durli
Everton Hiraiwa
Everton
Hiraiwa
Tulio Serrano
Tulio
Serrano
Geciele Rodrigues Teixeira
Geciele Rodrigues
Teixeira
Paulo Marcos Ribeiro Sampaio
Paulo Marcos Ribeiro
Sampaio
Marcus Vinicius Martins Collares
Marcus Vinicius Martins
Collares
Use of derived adipose stem cells to reduce complications of cutaneous scarring in smokers. An experimental model in rats
SciELO journals
2019
Stem Cells
Nicotine
Rats
2019-08-21 02:48:31
Dataset
https://scielo.figshare.com/articles/dataset/Use_of_derived_adipose_stem_cells_to_reduce_complications_of_cutaneous_scarring_in_smokers_An_experimental_model_in_rats/9697184
<div><p>Abstract Purpose To evaluate the use of adipose-derived stem cells (ADSC) in reducing the necrosis area in an experimental model of cutaneous ischemic flap in rats submitted to subcutaneous nicotine injection to simulate a smoker patient. Methods In an experimental study, 30 rats were enrolled and divided into two experimental groups of 15 animals all submitted to a subcutaneous nicotine injection to create ischemic cutaneous flaps on their backs. Other 10 animals were used only to obtain adipose tissue derived stem cells (ADSC). The first group (n=15) received ADSC treatment at the end of surgery while the other group, the control (n=15), received no other interventions. After euthanasia, a decal was performed on the whole area of the flap, accurately defining the transition from necrosis to healthy region. Photos of all animals were collected and evaluated by scales standardized by Paint-Autocad- 2015 software to define the area of flap necrosis in each rat. Student T test was performed to compare the groups, considering a p< 0.05 significant. Data were analyzed using SPSS IBM® 18 version. Results Through the analysis of the images by the program Paint-Autocad-2015 and the area of decal obtained by the transparent sheet, we obtained a mean of 46% necrosis of the total area of the flap in the treatment group and 69.4% in the control group. In the descriptive analysis, a mean of 3.7 cm of necrosis CI 95% (3.2 - 4.2) was evident in the treatment group whereas a mean value of 5.56 CI 95% (5.2 - 5.9) was found in control group, with p value <0.001 for this comparison. Conclusion The application of adipose-derived stem cells reduces the percentage of necrosis in an experimental model of randomized cutaneous flap in rats submitted to subcutaneous nicotine injection.</p></div>