10.6084/m9.figshare.9957116.v1 Elizangela Farias Elizangela Farias Fhabiane Bezerra Fhabiane Bezerra Djane Clarys Baia-da-Silva Djane Clarys Baia-da-Silva Yury Oliveira Chaves Yury Oliveira Chaves Tatiana Bacry Cardoza Tatiana Bacry Cardoza Maria Edilene Martins de Almeida Maria Edilene Martins de Almeida Lucas Barbosa Oliveira Lucas Barbosa Oliveira Pritesh Lalwani Pritesh Lalwani PatrĂ­cia Puccinelli Orlandi PatrĂ­cia Puccinelli Orlandi Marcus Vinicius Guimaraes Lacerda Marcus Vinicius Guimaraes Lacerda Stefanie Costa Pinto Lopes Stefanie Costa Pinto Lopes Paulo Afonso Nogueira Paulo Afonso Nogueira A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry SciELO journals 2019 Plasmodium vivax merozoites MSP1 opsonising antibodies 2019-10-09 02:45:33 Dataset https://scielo.figshare.com/articles/dataset/A_simple_ex_vivo_phagocytosis_assay_of_Plasmodium_vivax_merozoites_by_flow_cytometry/9957116 <div><p>As phagocytosis is the first line of defense against malaria, we developed a phagocytosis assay with Plasmodium vivax (P. vivax) merozoites that can be applied to evaluate vaccine candidates. Briefly, after leukocyte removal with loosely packed cellulose powder in a syringe, P. vivax trophozoites matured to the merozoite-rich schizont stages in the presence of the E64 protease inhibitor. The Percoll gradient-enriched schizonts were chemically disrupted to release merozoites that were submitted to merozoite opsonin-dependent phagocytosis in two phagocytic lines with human and mouse antibodies against the N- and C-terminus of P. vivax Merozoite Surface Protein-1 (Nterm-PvMSP1 and MSP119). The resulting assay is simple and efficient for use as a routine phagocytic assay for the evaluation of merozoite stage vaccine candidates.</p></div>