10.6084/m9.figshare.9957116.v1
Elizangela Farias
Elizangela
Farias
Fhabiane Bezerra
Fhabiane
Bezerra
Djane Clarys Baia-da-Silva
Djane Clarys
Baia-da-Silva
Yury Oliveira Chaves
Yury Oliveira
Chaves
Tatiana Bacry Cardoza
Tatiana Bacry
Cardoza
Maria Edilene Martins de Almeida
Maria Edilene Martins
de Almeida
Lucas Barbosa Oliveira
Lucas Barbosa
Oliveira
Pritesh Lalwani
Pritesh
Lalwani
PatrĂcia Puccinelli Orlandi
PatrĂcia Puccinelli
Orlandi
Marcus Vinicius Guimaraes Lacerda
Marcus Vinicius Guimaraes
Lacerda
Stefanie Costa Pinto Lopes
Stefanie Costa Pinto
Lopes
Paulo Afonso Nogueira
Paulo Afonso
Nogueira
A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry
SciELO journals
2019
Plasmodium vivax
merozoites
MSP1
opsonising antibodies
2019-10-09 02:45:33
Dataset
https://scielo.figshare.com/articles/dataset/A_simple_ex_vivo_phagocytosis_assay_of_Plasmodium_vivax_merozoites_by_flow_cytometry/9957116
<div><p>As phagocytosis is the first line of defense against malaria, we developed a phagocytosis assay with Plasmodium vivax (P. vivax) merozoites that can be applied to evaluate vaccine candidates. Briefly, after leukocyte removal with loosely packed cellulose powder in a syringe, P. vivax trophozoites matured to the merozoite-rich schizont stages in the presence of the E64 protease inhibitor. The Percoll gradient-enriched schizonts were chemically disrupted to release merozoites that were submitted to merozoite opsonin-dependent phagocytosis in two phagocytic lines with human and mouse antibodies against the N- and C-terminus of P. vivax Merozoite Surface Protein-1 (Nterm-PvMSP1 and MSP119). The resulting assay is simple and efficient for use as a routine phagocytic assay for the evaluation of merozoite stage vaccine candidates.</p></div>