A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry
Elizangela Farias
Fhabiane Bezerra
Djane Clarys Baia-da-Silva
Yury Oliveira Chaves
Tatiana Bacry Cardoza
Maria Edilene Martins de Almeida
Lucas Barbosa Oliveira
Pritesh Lalwani
PatrĂcia Puccinelli Orlandi
Marcus Vinicius Guimaraes Lacerda
Stefanie Costa Pinto Lopes
Paulo Afonso Nogueira
10.6084/m9.figshare.9957116.v1
https://scielo.figshare.com/articles/dataset/A_simple_ex_vivo_phagocytosis_assay_of_Plasmodium_vivax_merozoites_by_flow_cytometry/9957116
<div><p>As phagocytosis is the first line of defense against malaria, we developed a phagocytosis assay with Plasmodium vivax (P. vivax) merozoites that can be applied to evaluate vaccine candidates. Briefly, after leukocyte removal with loosely packed cellulose powder in a syringe, P. vivax trophozoites matured to the merozoite-rich schizont stages in the presence of the E64 protease inhibitor. The Percoll gradient-enriched schizonts were chemically disrupted to release merozoites that were submitted to merozoite opsonin-dependent phagocytosis in two phagocytic lines with human and mouse antibodies against the N- and C-terminus of P. vivax Merozoite Surface Protein-1 (Nterm-PvMSP1 and MSP119). The resulting assay is simple and efficient for use as a routine phagocytic assay for the evaluation of merozoite stage vaccine candidates.</p></div>
2019-10-09 02:45:33
Plasmodium vivax
merozoites
MSP1
opsonising antibodies