A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry Elizangela Farias Fhabiane Bezerra Djane Clarys Baia-da-Silva Yury Oliveira Chaves Tatiana Bacry Cardoza Maria Edilene Martins de Almeida Lucas Barbosa Oliveira Pritesh Lalwani PatrĂ­cia Puccinelli Orlandi Marcus Vinicius Guimaraes Lacerda Stefanie Costa Pinto Lopes Paulo Afonso Nogueira 10.6084/m9.figshare.9957116.v1 https://scielo.figshare.com/articles/dataset/A_simple_ex_vivo_phagocytosis_assay_of_Plasmodium_vivax_merozoites_by_flow_cytometry/9957116 <div><p>As phagocytosis is the first line of defense against malaria, we developed a phagocytosis assay with Plasmodium vivax (P. vivax) merozoites that can be applied to evaluate vaccine candidates. Briefly, after leukocyte removal with loosely packed cellulose powder in a syringe, P. vivax trophozoites matured to the merozoite-rich schizont stages in the presence of the E64 protease inhibitor. The Percoll gradient-enriched schizonts were chemically disrupted to release merozoites that were submitted to merozoite opsonin-dependent phagocytosis in two phagocytic lines with human and mouse antibodies against the N- and C-terminus of P. vivax Merozoite Surface Protein-1 (Nterm-PvMSP1 and MSP119). The resulting assay is simple and efficient for use as a routine phagocytic assay for the evaluation of merozoite stage vaccine candidates.</p></div> 2019-10-09 02:45:33 Plasmodium vivax merozoites MSP1 opsonising antibodies