10.6084/m9.figshare.9986354.v1 M.C.C. Andrade M.C.C. Andrade T.M. Moreno T.M. Moreno M.S. Muccillo M.S. Muccillo J.A.T. Pigatto J.A.T. Pigatto E.V. Camilo E.V. Camilo Evaluation of equine corneal endothelium after exposure to 0.05% brilliant blue - an in vitro study SciELO journals 2019 equine brilliant blue vital dye corneal endothelium 2019-10-16 03:00:57 Dataset https://scielo.figshare.com/articles/dataset/Evaluation_of_equine_corneal_endothelium_after_exposure_to_0_05_brilliant_blue_-_an_in_vitro_study/9986354 <div><p>ABSTRACT The aim of this study was to evaluate the immediate effects of 0.05% brilliant blue on corneal endothelium of horses. Thirty-eight corneas of 19 horses, male or female, of different ages were studied. Corneas were randomly divided into two groups. Group 1: Corneal endothelium was covered with 0.3mL of brilliant blue 0.05% for 60 seconds followed by rinsing with a balanced salt solution. Group 2: Corneal endothelium was covered with BSS for 60 seconds. The corneas were excised with an 8mm trephine and prepared to analyze posterior endothelial surface using a light microscope (24 corneas) and a scanning electron microscope (14 corneas). The equine posterior corneal endothelium surface observed by optical microscopy and scanning electron microscopy revealed a continuous layer of polygonal cells of uniform size and shape in both the control and treatment groups. Due to non-normal residuals at ANOVA mean comparison, a generalized linear model was utilized at 5% level of significance. The chi-square test stated that treatment and control group were not different statistically. The 0.05% brilliant blue did not cause damage to equine corneal endothelium.</p></div>