Chemo-characterization and optimization of macro and micro nutrients for exopolysaccharides and mycelia growth in pleurotus tuberregium (RUMPH, EX FR)
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Abstract Exoploysaccharides are potential nutraceutic, probiotic, phamarceutic and cosmesceutic natural products in fungi. Pleurotus tuberregium is an underutilized tropical fungus that is useful as food with recent reports of pharmacological activities. The present study evaluated and characterized environmental conditions and nutrients for optimal P. tuberregium mycelia growth (PtMG) and exopolysaccharides (PtEPS) production using batch culturing and diverse chemometric methods, respectively. Rank-sum analysis categorized the assessed conditions and nutrients into six stimulatory classes and most suitable nutrients and conditions for PtMG and PtEPS were co-identified with analysis of variance. Glucose, yeast, sodium chloride (NaCl) and tricalcium diphosphate (Ca3(PO4)2) were excellent stimulant (ES) for both PtEPS and PtMG under culturing condition of pH 6 at 30 °C for 15 d. The Chemo-phene trees generated by cluster analysis allowed visualization and clear-cut demarcation of the six stimulatory classes. The information embedded in the present study is useful for improving and developing culturing media for optimal PtMG and PtEPS yields, enabling maximal P. tuberregium utilization as food and medicine.