Determination of active compounds in raspberry leaf extracts and the effects of extract intake on mice

Abstract Twelve typical polyphenols in raspberry leaf powder (RLP), raspberry leaf extract 1 (RLE) and raspberry leaf extract 2 (RLE-H) were analyzed by HPLC-MS. The sum of their contents in three samples were in the following order: RLP (8.6 mg g-1) < RLE (35.0 mg g-1) < RLE-H (44.1 mg g-1). Ellagic acid and quercetin-3-O-glucuronide were the principal compounds in the samples and accounted for more than 75% of the sum content. A gelled mixture of RLE, pectin, and sodium alginate administrated to mice via gavage resulted in reduced weight gain and adiposity percentage, increased teste percentage, prolonged exhaustion time, furthermore alleviated intestinal flatulence possibly induced by RLE or RLE-H alone. These results could help in making rational use of this high polyphenol extract from raspberry leaves.