Effect of norbixin-based poly(hydroxybutyrate) membranes on the tendon repair process after tenotomy in rats

dataset

posted on 2020-01-15, 04:40 authored by Lízia Daniela e Silva Nascimento, Renata Amadei Nicolau, Antônio Luiz Martins Maia Filho, José Zilton Lima Verde Santos, Khetyma Moreira Fonseca, Danniel Cabral Leão Ferreira, Rayssilane Cardoso de Sousa, Vicente Galber Freitas Viana, Luiz Fernando Meneses Carvalho, José Figueredo-Silva

Abstract Purpose: To determine the efficacy of norbixin-based poly(hydroxybutyrate) (PHB) membranes for Achilles tendon repair. Methods: Thirty rats were submitted to total tenotomy surgery of the right Achilles tendon and divided into two groups (control and membrane; n = 15 each), which were further subdivided into three subgroups (days 7, 14, and 21; n = 5 each). Samples were analyzed histologically. Results: Histological analysis showed a significant reduction in inflammatory infiltrates on days 7, 14 (p < 0.0001 for both), and 21 (p = 0.0004) in the membrane group compared to that in the control group. There was also a significant decrease in the number of fibroblasts in the control group on days 7, 14 (p < 0.0001), and 21 (p = 0.0032). Further, an increase in type I collagen deposition was observed in the membrane group compared to that in the control group on days 7 (p = 0.0133) and 14 (p = 0.0107). Conclusion: Treatment with norbixin-based PHB membranes reduces the inflammatory response, increases fibroblast proliferation, and improves collagen production in the tendon repair region, especially between days 7 and 14.