Phenolic compounds from Virola venosa (Myristicaceae) and evaluation of their antioxidant and enzyme inhibition potential

posted on 12.12.2018 by Kamila Rangel Primo FERNANDES, Paulo Senna BITTERCOURT, Afonso Duarte Leão de SOUZA, Antonia Queiroz Lima de SOUZA, Felipe Moura Araújo da SILVA, Emerson Silva LIMA, Leonard Domingo Rosales ACHO, Rita de Cássia Saraiva NUNOMURA, Ana Frazão TEIXEIRA, Hector Henrique Ferreira KOOLEN

ABSTRACT Virola venosa, popularly known in Brazil as ucuuba-da-mata, occurs naturally in the Amazon region and has potential to provide useful natural compounds, as already known for other Virola species. Therefore, the objective of this study was to determine the chemical composition of bark and leaf extracts of V. venosa, and to test the antioxidant capacity and α-glucosidase inhibition potential of their compounds. Polar extracts showed to be more active in both assays, therefore a bioactivity-guided fractionation was performed to identify the compounds that were responsible for the recorded activities. Using a combination of LC-MS/MS analysis and isolation with NMR identification, eight phenolic compounds were identified. Assays with pure compounds of the active fraction revealed that ferulic acid was the main contributor compound to the observed bioactivity in the crude extracts.